The signification and characteristics of long steep slope section of rail transit lines, as well as the problems to be solved regarding the long steep slope section, are discussed. Simulation and engineering test made shows that the vehicle brake influence and the electrical motor heating occurring within a certain range of long steep slope section of rail transit lines are safe. The design is verified in the design of No. 4 line of Shenzhen Metro, which demonstrates that the measures taken for the long steep slope section are effective and rational.