The primary support, secondary lining and advanced support of support structure of large crosssectional tunnels on ZhengzhouWanzhou Highspeed Railway have disadvantages such as large emergency capacity and high cost. Hence, it is necessary to optimize the support parameters. The stress and strain of tunnel support structure of Hubei Section of ZhengzhouWanzhou Highspeed Railway are tested and analyzed based on general design drawing A, and optimization scheme of support structure is put forward. And then the optimization scheme is rechecked by loadstructure finite element method and field test method; and the optimized design drawing B is determined and applied to the project. The applicable results show that the support structure is in a safe state, which indicates the rationality of the scheme. By adopting the scheme, the safety and stability of large crosssectional tunnel can be guaranteed, the cost and working amount of labor can be reduced, and the material utilization rate can be improved.