The regular inspection behavior of highway tunnels should be standardized, and the regular inspection methods in the tunnel industry should be unified. As a result, the data investigation and statistical analysis are adopted to summarize the periodical inspection of 98 singlehole tunnels, the distribution characteristics of diseases according to the subitems of regular inspection, and the frequently occurring parts of tunnel diseases, and further to point out the key points for the maintenance department to carry out regular inspection work. And then, relying on the field investigation of the regular inspection of tunnels in recent years, a set of standardized methods for regular inspection of tunnels is put forward. The standardized description is made from the inspection plan, inspection tools, requirements, inspection results and countermeasures of the regular inspection of tunnels. The regular inspection links of highway tunnels are systematically sorted out in detail, and the norms for regular inspection are summarized to provide reference for maintenance units to carry out regular inspection work. Finally, based on the author′s many years of maintenance work experience, other nontechnical problems in the regular inspection work of tunnels are pointed out, which can provide reference for the improvement of the followup daily maintenance work of tunnels.