In order to further understand the information composition of the light environment of highway tunnels and discuss the mechanism of tunnel information perception under static and dynamic conditions, firstly, the latest research results on the information and perception of light environment of highway tunnels in China and abroad are summarized, and the substances that constitute the light environment of highway tunnels and the information they reflect are emphatically introduced. And then, the dynamic information perception in mancarroad driving environment is explored, and the static and dynamic information perception experiment is carried out. The results show that: (1) The light environment of highway tunnel includes direct and indirect factors. (2) The comprehensive information reflected by these factors can be divided into static and dynamic conditions. As a humanvehicleroad driving environment, the information perception involves both physical indicators (physical quantities) and human subjective responses, namely biological indicators (biomass). Finally, by analyzing and studying information perception mechanism, a dynamic information perception test platform is constructed and experimental research is carried out, and a research method and evaluation index of tunnel light environment information perception combining physical quantity and biomass interaction is preliminarily proposed.