Guanjiao tunnel is the longest high altitude tunnel in the world. 10 assistant inclined shafts are set for the tunnel and trackless transportation system is used in each inclined shaft. The ventilation of the inclined shaft work lots during tunnel construction is difficult. This paper aims to introduce the equipment layout, energy consumption analysis, false ceiling structure and air leakage prevention measures of inclined shaft partition method used for ventilation during the tunnel construction. The ventilation effect of the inclined shaft partition method used in the 10th inclined shaft work lot of Guanjiao tunnel demonstrates that the inclined shaft partition method can be promoted in the ventilation of inclined shafts with trackless transportation systems.