The 25km long tunnel with the circular crosssection on highspeed railway was constructed by TBM. Based on the characteristics and applications of the belt transportation and rail transportation, the transportation system in the 25km long tunnel was presented in this paper. In this system the track, equipment, train, rockdischarging, transportation organization, concrete transportation, transportation in the passage and the emergency transportation between the two parallel tunnels are elaborated, which provides reference to other similar projects.