To investigate the influence of high temperature and humidity environment on the health status of tunnel construction personnel, and formulate effective preventive measures in time, the physiological, subjective feeling, and behavioral indicators of 24 workers with various labor intensities are measured within 2 h of working in the No. 1 inclined shaft of the Gaolegongshan tunnel. The results reveal the following: (1) The change of heart rate of workers with light and medium labor intensity is less affected by the environment during normal working time, and the change of body index is within the tolerable range. (2) The heart rate tachycardia of workers with heavy and extremelyheavy labor intensity is much higher. (3) There is a positive correlation between the reaction time and the physical strength of the workers and the labor intensity. Within 2 h of working, the reaction time changes significantly in 80~100 min, while the physical strength changes more significantly in 100~120 min. For operation personnel with heavy and extremelyheavy labor intensity, it is necessary to control their continuous working time within 2 h and strengthen the supervision and management in the second hour.